Triclosan affects motor function in zebrafish larva by inhibiting ache and syn2a genes

The widespread use of triclosan in personal care products as an antimicrobial agent is leading to its alarming tissue-bioaccumulation including human brain. However, knowledge potential effects on the vertebrate nervous system still limited. Here, we hypothesized that sublethal concentrations are potent enough alter motor neuron structure and function zebrafish embryos exposed for prolonged duration. In this study, were used vertebrate-animal model. Prolonged exposure (up 4 days) 0.6 mg/L (LC50, 96 h) 0.3 (<LC50, Sublethal) produced aberrations innervations skeletal muscles reduced touch-evoked escape response larvae. This suggests dysfunction treated embryos. To further explore mechanisms induced neurotoxicity, determined enzyme activity acetylcholinesterase (AChE) expression (ache), myelin basic protein (mbp) synapsin IIa (syn2a) genes which play important role neural development synaptic transmission. ache syn2a down-regulated larvae without any significant changes mbp gene expression. At functional level, observed a decrease AChE activity. Furthermore, docking results showed can form stable interaction with binding pocket perhaps it compete natural acetylcholine direct thereby inhibiting affecting cholinergic Therefore, be regarded neurotoxic even at concentrations. Overall, growing toxicological evidence against ours suggest caution use.